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Bruker BioScope Catalyst AFM (bioAFM)


The Bruker BioScope Catalyst AFM is positioned on an inverted microscope, allowing for the integration of AFM imaging with optical imaging. This arrangement allows for the imaging of larger samples, including samples on glass slides or in glass bottom dishes. Our system is equipped with DAPI (blue), GFP (green) and CY3 (red) filter cubes as well as a large selection objectives (see below). For AFM imaging, the Catalyst has both PeakForce Tapping and the Nanomechanics software package, which allows the user to measure elastic modulus values and modulus maps for a wide range of materials, from the kPa to the GPa range.

Contact:
Julie Last
jalast@wisc.edu
608-262-3613




Rates: See fees page.  

Instrument details:

Axiovert 200 Microscope

X-Cite Mercury Arc Lamp with DAPI (blue), GFP (green) and CY3 (red) filters

Objectives:

1)    A-Plan 10x/0.25air, WD = 4.5  

2)    EC Plan-Neofluar 10x/0.3 air, WD = 5.2

3)    C-Apochromat 10x/0.45 water, WD = 1.8

4)    Plan Apochromat 20x/0.8,WD = 0.55

5)    C-Apochromat 40x/1.2water, WD = 0.28

6)    Fluar 40x/1.3 oil, WD = 0.16

7)    C-Apochromat 63x/1.2 water, WD = 0.28

8)    Plan-Apochromat 63x/1.4 oil, WD = 0.19

 

AFM Specifications

X-Y Scan Range ≥150μm, open-loop or closed-loop operation

Z Scan Range ≥20μm, open-loop or closed-loop operation

Deflection Detection IR super luminescent diode (SLD ), λ=850nm

Height Noise

<0.1nm RMS (air);

<0.2nm RMS (fluid)

(typical with appropriate vibration isolation)

Force Noise Thermally-limited, PicoForce-quality force measurements, <10pN RMS for cantilever with k=20pN/nm

XY Sample Stage

Motorized stage with 10x10mm range; includes magnetic sample clamps for 1x3in. slides, 25mm coverslips, 35 and 60mm plastic petri dishes, and 50mm glass bottom petri dishes

 

AFM Accessories

 MIRO Software

Allows real-time import and registration of optical and AFM images; optical images can be used to guide AFM imaging or force measurements; offline features allow image overlay, adjustment of colors and opacities, and flexible data export options

Perfusion Cells

Petri dish perfusion for 50mm glass-bottom petri dishes; Microvolume perfusion cell (<60μL cell volume); Perfusing Stage Incubator for long-duration live-cell research

Sample Heating

Enables operation up to 40°C in liquid; physiological temperature range imaging (up to 40°C)

Top-View Optics

Allows use with non-transparent samples while AFM remains mounted on the inverted microscope

Nanomechanics Package

PeakForce QNM, Quantitative Force-Volume Mapping; single force curves; and comprehensive suite of force curve analysis tools

 

Links:

Catalyst Experiment Guide

Bruker website

Probe selection guide

Peak force QNM

Peak force QNM with biological materials

Ċ
Julie Last,
Nov 20, 2013, 6:41 AM
Ċ
Julie Last,
Nov 19, 2013, 1:14 PM
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